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NHS Activated Silver Nanoparticle Kit (SN5K)

NHS Activated Silver Nanoparticle Kit (SN5K)

NHS activated silver nanoparticles designed for fast and convenient one-step conjugation reaction with no pre-activation requirements; ideal for development of protein silver conjugates for use in applications such as blotting, lateral flow assays, microscopy and transmission electron microscopy (TEM).

$182.00 — $490.00

conjugation directions

The NHS-activated Silver Nanoparticle Conjugation Kits have been optimized for high efficiency one-step conjugations of proteins and other primary amine-containing ligands to silver nanoparticles with diameters in the size range of 10nm-100nm.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the silver nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester silver nanoparticles supplied in the kit.

Kits are available in convenient 3 or 10 small-scale reactions formats allowing multiple to be conjugated simultaneously and ready for use in 2.5 hours or less. These kits are ideal for screening and optimization purposes prior to scale-up production. Scale up can be performed with our NHS-Activated Silver nanoparticles.

Features & Benefits

  • Results in covalently bound ligand and more stable conjugate.
  • Fast and convenient one-step conjugation reaction with no pre-activation requirements
  • Spacer between the silver nanoparticle surface and conjugated ligand minimizes effects on the tertiary protein structure, which can lead to poor performing conjugates, which is a common problem seen in conjugates prepared by passive adsorption.

Applications

  • Ideal for development of protein silver conjugates for use in applications such as blotting, lateral flow assays, microscopy and transmission electron microscopy (TEM).

Kit Components

  • NHS-Activated Silver Nanoparticles (lyophilized)
  • Protein Re-suspension Buffer
  • Reaction Buffer
  • Quencher Solution

Technical Specifications | Peer Reviewed Publications

Catalog No. Item/Description
SN5K-10-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 10nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 120ug of protein. ($182)
SN5K-10-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 10nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 120ug of protein. ($490)
SN5K-10-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 10nm silver nanoparticles. Contains enough reagents for conjugation of 1.2 mg of protein. ($490)
SN5K-20-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 20nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 40ug of protein. ($182)
SN5K-20-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 20nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 40ug of protein. ($490)
SN5K-20-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 20nm silver nanoparticles. Contains enough reagents for conjugation of 400ug of protein. ($490)
SN5K-30-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 30nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 40ug of protein. ($182)
SN5K-30-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 30nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 40ug of protein. ($490)
SN5K-30-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 30nm silver nanoparticles. Contains enough reagents for conjugation of 400ug of protein. ($490)
SN5K-40-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 40nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 20ug of protein. ($182)
SN5K-40-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 40nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein. ($490)
SN5K-40-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 40nm silver nanoparticles. Contains enough reagents for conjugation of 200ug of protein. ($490)
SN5K-50-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 50nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 20ug of protein. ($182)
SN5K-50-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 50nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein. ($490)
SN5K-50-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 50nm silver nanoparticles. Contains enough reagents for conjugation of 200ug of protein. ($490)
SN5K-60-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 60nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 20ug of protein. ($182)
SN5K-60-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 60nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein. ($490)
SN5K-60-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 60nm silver nanoparticles. Contains enough reagents for conjugation of 200ug of protein. ($490)
SN5K-80-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 80nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 20ug of protein. ($182)
SN5K-80-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 80nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein. ($490)
SN5K-80-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 80nm silver nanoparticles. Contains enough reagents for conjugation of 200ug of protein. ($490)
SN5K-100-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 100nm silver nanoparticles. Contains enough reagents for conjugation of 3 x 20ug of protein. ($182)
SN5K-100-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 100nm silver nanoparticles. Contains enough reagents for conjugation of 10 x 20ug of protein. ($490)
SN5K-100-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 100nm silver nanoparticles. Contains enough reagents for conjugation of 200ug of protein. ($490)