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NHS Activated Gold NanoUrchins (GUN10K)

NHS Activated Gold NanoUrchins (GUN10K)

NHS-activated Gold NanoUrchin Conjugation Kits optimized for high efficiency one-step conjugations of proteins and other primary amine-containing ligands to gold nanoparticles with diameters in the size range of 5nm-100nm. Ideal for development of protein gold conjugates for use in applications such as blotting, lateral flow assays, microscopy and transmission electron microscopy (TEM).

$182.00 — $490.00

conjugation directions
Non-functionalized Gold NanoUrchins have unique optical properties compared to spherical gold nanoparticles of the same core diameter. The spiky uneven surface causes a red shift in the surface plasmon peak and a larger enhancement of electromagnetic fields at the tips of the Gold NanoUrchin spikes compared to that of a spherical particles. As an example, 100nm spherical gold nanoparticles have an SPR peak at 570nm while 100nm Gold NanoUrchins have a SPR peak at around 680nm. In addition, binding of ligands such as proteins to the Gold NanoUrchin surface causes a larger shift in the surface plasmon peak compared to standard spherical gold nanoparticles.

The kit contains ready-to-use pre-made mixtures. No activation or manipulation of the gold nanoparticles is required prior to conjugation, which often results in poor performing conjugates. Simply mix your protein with the pre-activated NHS ester gold nanoparticles supplied in the kit.

Kits are available in convenient 3 or 10 small-scale reactions formats allowing multiple to be conjugated simultaneously and ready for use in 2.5 hours or less. These kits are ideal for screening and optimization purposes prior to scale-up production. Scale up can be performed with our NHS-Activated Gold.

Peer Reviewed Publications

Catalog No. Item/Description
GUN10K-50-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 50nm Gold NanoUrchins. 50nm Conjugation Kit (3rxn X 10ug of protein), 585nm absorption peak, OD=20 at 100uL after conjugation ($165)
GUN10K-60-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 60nm Gold NanoUrchins. 60nm Conjugation Kit (3rxn X 10ug of protein), 585nm absorption peak, OD=20 at 100uL after conjugation ($165)
GUN10K-70-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 70nm Gold NanoUrchins. 70nm Conjugation Kit (3rxn X 10ug of protein), 600nm absorption peak, OD=20 at 100uL after conjugation ($165)
GUN10K-80-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 80nm Gold NanoUrchins. 80nm Conjugation Kit (3rxn X 10ug of protein), 620nm absorption peak, OD=20 at 100uL after conjugation ($165)
GUN10K-90-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 90nm Gold NanoUrchins. 90nm Conjugation Kit (3rxn X 10ug of protein), 630nm absorption peak, OD=20 at 100uL after conjugation ($165)
GUN10K-100-1 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 100nm Gold NanoUrchins. 100nm Conjugation Kit (3rxn X 10ug of protein), 680nm absorption peak, OD=20 at 100uL after conjugation ($165)
GUN10K-50-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 50nm Gold NanoUrchins. 50nm Conjugation Kit (10rxn X 10ug of protein), 585nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-60-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 60nm Gold NanoUrchins. 60nm Conjugation Kit (10rxn X 10ug of protein), 585nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-70-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 70nm Gold NanoUrchins. 70nm Conjugation Kit (10rxn X 10ug of protein), 600nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-80-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 80nm Gold NanoUrchins. 80nm Conjugation Kit (10rxn X 10ug of protein), 620nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-90-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 90nm Gold NanoUrchins. 90nm Conjugation Kit (10rxn X 10ug of protein), 630nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-100-2 One-Step mini kit for covalent conjugation of proteins and other amine containing ligands to 100nm Gold NanoUrchins. 100nm Conjugation Kit (10rxn X 10ug of protein), 680nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-50-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 50nm Gold NanoUrchins. 50nm Conjugation Kit (1rxn X 100ug of protein), 585nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-60-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 60nm Gold NanoUrchins. 60nm Conjugation Kit (1rxn X 100ug of protein), 585nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-70-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 70nm Gold NanoUrchins. 70nm Conjugation Kit (1rxn X 100ug of protein), 600nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-80-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 80nm Gold NanoUrchins. 80nm Conjugation Kit (1rxn X 100ug of protein), 620nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-90-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 90nm Gold NanoUrchins. 90nm Conjugation Kit (1rxn X 100ug of protein), 630nm absorption peak, OD=20 at 100uL after conjugation ($445)
GUN10K-100-3 One-Step MIDI scale-up kit for covalent conjugation of proteins and other amine containing ligands to 100nm Gold NanoUrchins. 100nm Conjugation Kit (1rxn X 100ug of protein), 680nm absorption peak, OD=20 at 100uL after conjugation ($445)